Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.

نویسندگان

  • Panagiotis Karanis
  • Oriel Thekisoe
  • Klytaimnistra Kiouptsi
  • Jerry Ongerth
  • Ikuo Igarashi
  • Noborou Inoue
چکیده

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 73 17  شماره 

صفحات  -

تاریخ انتشار 2007